Guide Design Resources
Thank you to the thousands of users who visited our guide design tool over the past five years. We recently shut down crispr.mit.edu, but there are many other guide design tools available that we hope you will find helpful.
Benchling
Broad Institute GPP
CasOFFinder
CHOPCHOP
CRISPOR
Deskgen
E-CRISP
Geneious (not cloud based)
Guides (for library design)
Horizon Discovery
IDT
Off-Spotter
Synthego
TrueDesign (ThermoFisher)
Addgene blog post
Wiki on Cas9 guide design